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noncancerous human fibroblast lung cell line  (ATCC)


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    ATCC noncancerous human fibroblast lung cell line
    Noncancerous Human Fibroblast Lung Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5606 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noncancerous human fibroblast lung cell line/product/ATCC
    Average 99 stars, based on 5606 article reviews
    noncancerous human fibroblast lung cell line - by Bioz Stars, 2026-02
    99/100 stars

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    Direct effect of dextrose exposure on fibroblast metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.

    Journal: Cartilage

    Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

    doi: 10.1177/19476035251408601

    Figure Lengend Snippet: Direct effect of dextrose exposure on fibroblast metabolic activity immediately following treatment. XTT assay, displaying optical density (OD) readings of fibroblasts treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, run in duplicate, n = 5, error bars represent SEM, * P <0.05.

    Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

    Techniques: Activity Assay, XTT Assay, Control

    Direct effect of dextrose exposure on fibroblast metabolic activity 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 48 hours prior to running the XTT assay, displaying optical density (OD) readings, run in triplicate, n = 5, error bars represent SEM, * P < 0.05.

    Journal: Cartilage

    Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

    doi: 10.1177/19476035251408601

    Figure Lengend Snippet: Direct effect of dextrose exposure on fibroblast metabolic activity 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 48 hours prior to running the XTT assay, displaying optical density (OD) readings, run in triplicate, n = 5, error bars represent SEM, * P < 0.05.

    Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

    Techniques: Activity Assay, Control, XTT Assay

    Percent change in fibroblast metabolic activity comparing immediately following dextrose treatment to 48 hours following treatment. MRC-5 fibroblasts were exposed to concentrations of 5%, 10%, 15%, 20%, or 25% dextrose for 15, 30, 60, or 120 min. Absorbance was measured by XTT assay immediately following these treatment conditions and then also 48 hours following each of these treatment conditions. Average absorbance values using the XTT assay were calculated for each type of treatment: (1) immediately following direct treatment with dextrose and (2) 48 hours following direct treatment. The percent change was calculated as a ratio of the 48-hour average absorbance value minus the immediate average absorbance value divided by the average absorbance value immediately following treatment for each matched dextrose concentration and exposure time. These values were normalized to the corresponding media control for each duration of dextrose treatment (15, 30, 60, or 120 min). Averaged values represent 5 independent experiments.

    Journal: Cartilage

    Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

    doi: 10.1177/19476035251408601

    Figure Lengend Snippet: Percent change in fibroblast metabolic activity comparing immediately following dextrose treatment to 48 hours following treatment. MRC-5 fibroblasts were exposed to concentrations of 5%, 10%, 15%, 20%, or 25% dextrose for 15, 30, 60, or 120 min. Absorbance was measured by XTT assay immediately following these treatment conditions and then also 48 hours following each of these treatment conditions. Average absorbance values using the XTT assay were calculated for each type of treatment: (1) immediately following direct treatment with dextrose and (2) 48 hours following direct treatment. The percent change was calculated as a ratio of the 48-hour average absorbance value minus the immediate average absorbance value divided by the average absorbance value immediately following treatment for each matched dextrose concentration and exposure time. These values were normalized to the corresponding media control for each duration of dextrose treatment (15, 30, 60, or 120 min). Averaged values represent 5 independent experiments.

    Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

    Techniques: Activity Assay, XTT Assay, Concentration Assay, Control

    Indirect effect of dextrose exposure on the metabolic activity of nascent fibroblasts 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 8 hours prior to collecting the supernatant fluid. Nascent fibroblasts (not exposed to dextrose) were then incubated with this supernatant fluid from dextrose-treated cells for 48 hours. XTT assay, displaying optical density (OD) readings of nascent fibroblasts 48 hours after being exposed to supernatant fluid taken from fibroblasts directly treated with dextrose, run in triplicate, n = 5, error bars represent SEM. * P < 0.05.

    Journal: Cartilage

    Article Title: In Vitro Model Exploring the Mechanisms of Dextrose Prolotherapy: Fibroblasts Exposed to Clinical Concentrations of Dextrose Exhibit Significant Rebound Effects 48 Hours After Exposure

    doi: 10.1177/19476035251408601

    Figure Lengend Snippet: Indirect effect of dextrose exposure on the metabolic activity of nascent fibroblasts 48 hours after treatment. After fibroblasts were treated with 5%, 10%, 15%, 20%, and 25% dextrose compared to media control for 15, 30, 60, and 120 minutes of exposure, the supernatant fluid was replaced with fresh media and the cells were maintained at 37°C, 5% CO 2 for 8 hours prior to collecting the supernatant fluid. Nascent fibroblasts (not exposed to dextrose) were then incubated with this supernatant fluid from dextrose-treated cells for 48 hours. XTT assay, displaying optical density (OD) readings of nascent fibroblasts 48 hours after being exposed to supernatant fluid taken from fibroblasts directly treated with dextrose, run in triplicate, n = 5, error bars represent SEM. * P < 0.05.

    Article Snippet: The MRC-5 cells, a human lung fibroblast cell line (ATCC; cat# CCL-171), were cultured in complete medium made from Eagle’s Minimum Essential Medium (ATCC; cat# 3002003) supplemented with 10% fetal bovine serum (Atlanta Biologicals; cat# S11150 ), 1% penicillin/streptomycin (ATCC; cat# 30-2300), and 2 mM L-glutamine (ATCC; cat# 30-2214) according to the protocol for subculture recommended by ATCC.

    Techniques: Activity Assay, Control, Incubation, XTT Assay

    Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

    Journal: Microbial Cell Factories

    Article Title: Recombinant L-asparaginase from Stenotrophomonas maltophilia : a promising low-immunogenic anticancer agent

    doi: 10.1186/s12934-025-02856-0

    Figure Lengend Snippet: Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

    Article Snippet: Human leukemia cancer cell line K-562, human hepatocellular carcinoma cell line HepG-2, and normal human lung fibroblast cell line MRC-5 were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

    Techniques: Activity Assay, Recombinant, Produced, Standard Deviation